a plastic ring and disc. In each case the weight of the wet sample was estimated to
the nearest 10th gram, on a Scripps electronic shipboard balance. The potassium
activity of plankton is at most 5 d/min/g of wet material and should be considerably
lower. No correction was made for this natural activity.
One-half of the original sample was retained by Scripps for biological examination and one-quarter was taken by the University of Washington for activity measurement in their laboratories,
Analyses of Fish Samples
Fish samples were identified and dissected when necessary by the marine biologist. Samples were ashed at 600°C in nickel crucibles and weighed portions of the
ash taken for analysis. The ash was slurried with about 500 ml of dilute sodium carbonate solution in an attempt to remove the natural potassium activity and the residue
filtered off on fibre glass. The samples were covered with Mylar and mounted on
plastic rings and discs for counting.
When treated samples were analyzed by HASL by beta absorption techniques, it
was apparent that the major portion of the activity was due to the natural potassium in
most cases, This indicated poor separation by the method described and a number of
samples were reanalyzed by dissolving the ash and reprecipitating carbonates. In addition, several samples were analyzed for individual fission products at the HASL
laboratory.
Samples of all reef fish, flying fish and other biological specimens were taken
back to the Applied Fisheries Laboratory for activity measurement. There, individual
tissues were ashed and counted,
Counting Procedures
The beta counters described had an average background count of 18 to 20 cts/min.
The procedure on all samples consisted of running alternate periods of 30 min on
background and 60 min on the sample. The average background for the period before
and after the sample reading was subtracted from the sample count to give the net
count. The efficiency of the counters was measured with half-gram samples of potassium carbonate mounted and measured under the same conditions as the sample ex‘cept that a 10-min counting period was used. The K49 activity was taken as
492 d/min/half-gram. This standardization is reasonable for mixed fission products,
since the average energies of K#9 and of MFP are very close. The average efficiency
of the counters was 20 percent.
The counting statistics of all samples of one type are roughly equivalent.
For
water samples, the Poisson standard deviation is one count per minute per sample
which becomes 25 d/min/liter. Plankton and fish samples were weighed, and in most
cases, one gram samples were taken. This gives a standard deviation of 5 d/min/g
unless stated otherwise in the results.
The only possibility of improving the counting statistics is the development of low
background counters. It is not possible to handle larger samples of material nor is a
counting time greater than one hour practical for field use. If a 2-in. diameter Geiger
tube with a background of 10 cts/min were available, the standard deviation on a water

sample would be reduced to 15 d/min/liter while a 5-cts/min background would reduce

the standard deviation to 10 d/min/liter. It is apparent that even a marked background
improvement does not eliminate the problems of low level counting. A reliable
estimate is obtained only by combining results ona relatively large number of
samples,
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