5

,

Gk;
a

are

a

the coral used was without the cil scum and had noe count above

background on the survey meter,

The fish in #6 has likewise fed

regularly.

Aquaria #1, 3 and 4 were retained as controls,
Six larger aquaria, 12" x 24" x 16", were set up originally
but were not as satisfactory as the setup for the smaller aquarina

described above. The large aquaria are now being used as supply
tanks for fish and other marine animals as well as for an irradiation
mortality experiment with the marine snail, Thais lamellosa,
Qn April 14th mix more of the smaller aquariawere delivered and
were set up in the same manner as those for the flatfish.

Tide

Pool johmnies, a soulpin, Oligocuttus maculosus, were placed in these
aquaria,

They have not yet been used experinentally but seem well

adapted to their new environment,
The experiment in aquaria #2 and #5 will be continued until it is

believed that the fish is about to die,

At that time tissues will be

praserved for histological studies,

Project VI.

Observations on the amphipods were discontinued because of

high mortalities among the controls.

Marine snails, Thais lamellosa,

A report will be submitted.

that had been surviving well

in 15-gallon aerated aquaria, were individually marked with a number
and X-rayed on April 2.

Exposures given, and numbers of snails

irradiated, weret 10,000 r —- 23 S000 r - 63 2000 r=93
500 r= 93 and 200 r- 9,

1250 r ~ 9;

After 3 weeks the mortalities anong irra-

diated and control snails were approximately equal.

The snails

spawned in the aquaria, attaching their ogg cases to rocks, shells,

and especially to the glass aides of the aquaria.

When only a few

egg oases were deposited, their positions were recorded along with
ve

DosARCHIVES

Select target paragraph3