18
Marshallese more nearly resemble the blood

groupingsof the people of the Western Islandsof
Indonesia than those of the Amerindians. Too
great a generalization of population origin, however, cannot be drawn from sucha relatively small

study group.
Haptoglobin studies. Results of the haptoglobin
studies are taken from a report of Blumberget al.”
With the starch gel method of electrophoresis
there are three distinct patterns of haptoglobins."

In type 1 —1, there is one haptoglobin travelling

near the beta globulin region; in type 2 —2, there
are three haptoglobins travelling between the
alpha and beta globulins; and in type 2—1, there

are four haptoglobins. Oneof these appears to be
the same as in type 1—1 and the other three

migrate slightly further than the three haptoglobins
of type 2—2. It has been shownthat these hapto-

globin patterns are genetically determined, and
they also appear to have a physiological function
associated with hemoglobin transport.**-*9
Occasionally an individual is found who has no
haptoglobin at all. It is not yet clear if this type
is genetically or environmentally determined. In
the Marshallese, one such individual was found,

and he was excluded in computing the phenotype
frequencies. The actual incidenceof the haptoglobin types wasas follows.
Tyre

No.

%

1-1
2-1
2-—2
0-0

51
54
20
1

40.48
42.86
15.87
0.79

126

100.00

Total

The Marshall Islanders have a very high incidence of the | —1 type of haptoglobin, and incidence of the H,' gene exceeded only by that of
the Yorubas of Nigeria.*° The Alaskan Eskimos
have a low incidenceof this type. There appears
to be a primafacie relationship with latitude; populations from areas near the equator havea high
incidence of | —1 and a low incidence of 2—2,

personsin high latitudes the reverse, and populations from temperate climates intermediate values.
Thereis, of course, no evidence that the relation-

ship is more than coincidental. Anotherpossible
correlation is diet; the Artic people live primarily
on a protein diet (sea mammals, fish, and caribou),

while the Nigerians have a diet with a high carbohydrate content. Further studies are required to
confirm or reject this association. The hapto-

globin system represents one of the best examples
of genetic polymorphism yet described in humans.
A studyofits incidence in various people may help
in elucidating the selective factors that maintain
its balance in the population.
Hemoglobin types.

In the 45 Marshallese

blood samples analyzed, hemoglobin A, was found
to be a little more diffuse than in the control samples. There was also an increased smearingofthe

hemoglobin behind the A, zone. However, the

hemoglobins were considered to be normal, and
the abovefindings were believed to be due to possible denaturation which caused increased diffusion and smearing of the hemoglobin during
electrophoresis.
Serological and Urine Studies.

Serum proteins.

Asnotedin the past, the serum protein levels were
elevated in both exposed and unexposed people.
The meanlevel of the exposed group was 7.6,
and of the unexposed group 7.8 g. Electrophoretic
studies last year showed the gammaglobulin to be
elevated.
Tests of thyroid function.

The following was

reported by Dr. J.E. Rall. The level of serum
protein-boundiodine in both groupswassignificantly above the normal range (see Table 9). The
Normal PBI in man in the United States by this
technique ranges from 3.5 to 7.5 ug%. There appeared to be no correlation of PBI with either age

or sex. The ages of those examined ranged from
6 to 83. The total iodine of these sera averaged
1.0 ug% above the PBI, which is a normal value

and anindication that contamination with iodine
was not contributing to the elevated PBI values.
To determine whether the PBI mightconsist of
other organic but notthyroidaliodine, butanol
extractable iodine tests were performedon6 sera.
These values were at the upperlimit of normal
(normal range 3.2 to 6.4 ug%). To delinate further

these findings, thyroxine-binding capacity studies

were performed on selected sera by methods

previously described.’ In 12 cases the values
averaged 0.26 pg thyroxine/ml serum and ranged
from 0.186 to 0.32. The normal value is 0.20
pg/ml, but inadequate data are available to define
precisely whether the values in these Marshallese

individuals were significantly different from nor-

mal. It can, however, be calculated that the level

of thyroxine-bindingprotein is insufficient to cause
an elevation of serum thyroxine (presumably to
maintain a normallevelof free thyroxine) as seen
in these subjects. Contamination ofthe sera with

Select target paragraph3