AOL 31 11/24/73 SUMMARY ON ADVICE CONCERNING MNIKK BIKINT ISLANDERS Brewen and Preston: Oak Ridge Logistics: 200 people, 6 per day - would take two people about 1 month Equipment: Small med lab, 2 portable incubators, centrifuge, microscope ‘People: perhaps 1 person could do it, but two much better need people of technician level - highly qualified Carolyn Gooch could do it, Judy Bodicoat (Shelly's assistant) could tox Siside reading: More than 1 lab might do it Shelly Wolf Mike Bender ” re 7 é Brewen and Preston "~~ Kastenbaum-~--Amer: -Tob+ Inst--Statistician-and expert on experimental design Medical records: Good records needed Organization: perhaps need an expert panel to get it organized. Mayall and Carrano: Lawrence Livermore Method: Interested in using their technique of measuring total DNA /chromosome Very sensitive now. looks promising and need a test population to work it out on. with age. Perhaps can see a 2 to 5% difference Perhaps 18 months until ready to go in field mitn DNA variation is indeed greater in older people, say in the BikdidkdGink content of a specific chromsome. or gaps Chromsomal method - loss: let culture go through 2 divisions, which would show up---as a DNA-loss in-2nd. division. Blood could be collected in field - delivered to Livermore. Chromosomes would be stained with usual method and also banding methods. Semen samples might also be collected -- Bart Gladhill, reproductive biologist Johns Hopkins Shoudd do itt} Method Blood samples pre-resettlement - somebody in Hawaii could make slides. Fly blood to Hawaii. fimmm Slides could be stored for examination later - should be frozen, but important to be kept dry ' People: A ¢professional should plan it and decide who whould do it. farrano - good, but might like to practice first In house capanility - perhaps faster; LASL - Peterson, Devin (worked with Brandom) fo ae <— Pm Oak Ridge. or) = Bender: