Pa
TTTNT]
]
1000
ba
35
lOO
mg
°
°
rp Plasma
‘
°
PTT
Percent Injected Dose Per Gram Calcium
E
E
x
r Whole body
- retention
Dog
A22B
Ozage 8 mt. Bg
x
xsage 9 yr.
“Ca
ne
i
Oe eo, «+
hy
.
Tee
.
10k
10“
°
E
po*L
.01
0
al
wt
pt
1.0
et
10
100
TIME IN DAYS
1000
Iria. 33.—The plasma specific activity and the whole body retention of “*Ba and **Ca given intravenously into a single beagle
¢ dow, but at two widely different ages. “Ba was administered when the animal was 8 months of age and “{Ca when the animal was
inction
9.2 vears of age.
n and
vs. 8.5
aurves
J2 to
3 and
I(T)
‘ed in
ations
from
ected
TOXIe dog
oring
1 for
were
m to
s for
ormi576.
reent
itted
Tum,
Dogs
allafrom
3 ml samples of whole blood were dissolved in liquid
scintillation vials using hydroxide of hyamine and
10 ml each of absolute ethanol and scintillation solu| tion. The vials were then counted against aliquots of
the *%Ba injection solution (dissolved in the same
| manner as the plasma) in a Packard Tri-Carb liquid
scintillation spectrometer system, Model 3002. Quench-
ing was controlled by gain adjustments and obser-
vations of the spectrum shift through simultaneous
two-channel counting with one narrow and one wide
window opening. These observed values are also listed
in Table 9 and plotted in Figure 32.
Urine Samples
Urine samples were wet ashed with concentrated
HNOs and then diluted up to volume in volumetric
flasks. Three-milliliter aliquots from each sample
taken from Dog 576 or A22B were sealed in glass
vial and counted in the same Nal(Tl) well counter
muffle furnace, dissolved in concentrated HNOs, trans-
ferred to volumetric flasks, and diluted to volume. Ali-
quots from the prepared samples from the appropriate
dog were either sealed in glass vials and gamma
counted in the well counter or dissolved in liquid scintillation solution and counted in the liquid scintillation
spectrometer. All fecal and urinary excretion rates
(percent of injected dose per day) are summarized in
Table 10.
Bone Determinations
The bone specimens were air dried, weighed, placed
in separate preweighed crucibles, ashed overnight in a
muffle furnace at 750° C, reweighed to determine the
ash weight of each sample, dissolved in 2N HCl, and
then transferred to volumetric flasks. Five long bones
(a femur, tibia, humerus, radius, and ulna) from Dog
576 and the left femur from each of the other dogs
aliquots of the wet ashed urine samples from Dogs
were cut into five pieces, which were coded as proximal
end, proximal shaft, mid-shaft, distal shaft, or distal
tion vials and counted in the liquid scintillation coun-
other samples, appropriate aliquots were either sealed
that was used for the plasma clearance studies. Smaller
A22C and A22D were transferred to liquid seintillater,
Fecal Samples
All fecal samples were placed in separate porcelain
crucibles, heat dried, ashed overnight in a 600°C
end prior to weighing and ashing. As was done with the
in glass vials and counted in the well counter or dis-
solved in scintillation solution and counted in the liquid
scintillation counter. The observed values, listed in
Table 11, were calculated by comparison with the
standards made from theoriginal injection solution.