Provide subjecre with a one-liter or larger plastic botrle which
has been pre-treated with thymol preservative.
Note subjects name,
location,
date and time on sample bottle.
Instruct subject to void
and empty bladder just before beginning sample collection, and co
wash hands before each successive voiding inco the sample concainer.
Collece all urine for the nexe 24 hours in the sample concainer,
including a final voiding to empty che bladder just before returning
the container to the field-trip team or its representative.
Note
date and tine of final voiding.
The same container may be used for single-void samples. Ask
subject to wait until he or she has to urinate, wash hands, then
void into concainer until bladder is empty.
(2)
Larzge-volume samoles
Provide subjece with a 24 gallon or 5 gallon “cubitainer" or similar plastic container which has been pre-treated with thymol.
Note
subject's name,
location,
dace and rime on container.
Instruct subject
to void and empty bladder just before beginning sample collection, and
to wash hands before each successive voiding into the sample container.
Collect all urine for the next 120 hours (5 days) or longer if possible
(maximum:
10 days).
Just before returning the container to the fieldtrip team ac the end of the sampling period, the bladder should be
empcied in one final voiding.
Note the date and cime of the end of the
sampling period on the container.
D.
Sample Container Preparation, and Post-Collection Treatment
All sample containers should be "pre-treated" by adding 15 ml of 10%
thymol solution in alcohol.
The solution should be swirled in the conrainer
to completely coat the sides, and the top should be left off until the alcohol evaporates leaving a dry chymol residue coacing its inner surfaces.
After sample collection, 10 ml of concentrared HNO3 should be added to
each container per liter of urine collected.
Sample volume may be estimated
The amount (volume) of HNO3 added and date should be noted on the sample
container.
The container may then be sealed and packed for shipmenc to BNL.
Upon arrival at BNL, the sample volume and pH should be measured, and
additional coneentrated HNO3 added to adjusce the pH to ~2.0. The samples
may then be submitted for analysis.