AOZGOO
Hf.
November 30, 1962
Leo M. Meyer, M.D.
South Nassau Communities Hospital
2445 Oceanside Road
Oceanside, New York
Dear Leo,
Here is the procedure on the use of tritiated water for
measuring total body water.
1.
We will provide the HTO.
Let me know by return mail if we
should send it to you and where, or if you will pick it up when
you pass through San Francisco,
2.. Give by mouth 1 cc of RTO.
f
Transfer from stock bottle to
a glass, cup, or beaker exactly 1.00 ce HTO with a tuberculin
resectSPINES, (OF 1.6. pipette).
Add.50r100 oo water: and HAVASUbe wees
jeet drink entire contents.
Add another 50-100 cc water to the
vessel and again have subject drink entire contents.
36: Tritium dilution can be determined in either plasma or urine
(or both).
! If urine samples are collected, follow this procedure:
(a)
Have subject void about 4 hrs. after taking tritium. (b)
Take 10 ee urine samples at approximately 5 hrs., 8 hrs., and
12!nrs. after taking tritium.
can be obtained.
A 24 hr. sample is useful if tt
Put the urine sample in a 3 or 5 dram vial
with a erystal of thymol and labeled with name or code number,
date and time after administration of HTO.
: If a plasma sample is taken (in addition to or instead of
urine), take the sample 4+ to 6 hrs. after giving the tritiun.