controls, although two hemoglobin samples, 6A and 8A,
from the group of 9 indi-
viduals exposed to 69 R had high contents of isoleucine.
Table 1.
Frequency of substitution of isoleucine for other amino acids
in human hemoglobin from Marshallese.
Exposure (R)-
No. of
samples
0 controls
25
69
175 in utero
69 in utero
9
3
1
175
O offspring
Substitution
frequency + SEM (x 10°)
3.09 + 0.33
39
4.77 + 0.67
5
5.24 + 0.85
p value*
.
4.45 + 1.16
3.26 + 0.64
6.84
0.05>p>0.025
NS
NS
NS
NS
*Probability of significant difference from controls.
Blood samples were obtained from 9 exposed persons in successive years
to determine whether fluctuations in isoleucine content occurred. The data
are shown in Table 2. Fluctuations noted for samples 3R, 24R, 35R, and 71R
could be due to variant clones of erythrocytes arising from individual stem
cells, which appear and disappear from the peripheral circulation. Such fluctuations have not been observed in specimens from normal Laboratory personnel.
Table 2.
Frequency of substitution of isoleucine in samples analyzed in
consecutive years.
Substitution frequency
Exposure (R)
175
175
175
175
175
69
69
69
69
Sample number
3R
1974
19.79
24R
33R
35R
71R
6A
41A
454A
13.45
4.74
5.19
8.29
6.98
4.04
454
3.65
- 34 -
(x 105)
1975
1976
1.76
6.69 .
7.00
2.16
2.17
1.45
6.29
2.56
2.17
3.23
2.85
3.10