SMS RADIOACTIVITY OF MARINE ORGANI FROM GUAM, PALAU AND THE GULF OF SLAM, 1958-1959 both the test site (Eniwetok and Bikini) and two of the collecting areas (Guam and Palau) are within the path of the westward-flowing part of this cur- ALLYN H. SEYMOUR The arrival of Hardtack fallout at the collecting areas was determined indirectly rather than by a direct measurement of the radioactivity in sea water. The criterion was a significant increase rent system (see Figure 1). Laboratory of Radiation Biology, University of Washington, Seattle, F ashington in the gross beta activity of marine organisms. There ere two reasons for using this criterion: first, small amounts of radionuclides in sea water INTRODUCTION are difficult to detect in the presence of the salts that are normally present; and, secondly, Following the Operation Hardtack (Phase I) puciear test The collecting area in the Gulf of Siam is 4,250 miles west of Eniwetok but outside the North Equatorial Current. biological series at Bikini and Eniwetok Atolls in 1958. a study was made to measure changes with time in the radioactivity in marine organisms collected at stations located at distances of over one organisms concentrate certain fallout nuclides and thus the specific activity for them is higher than for sea water. For these reasons bio- logical samples are easier to prepare for counting thousand miles from the test site. The radionuclides of principal interest were those from local and require less counting time than sea water samples. currents from near the test site to the collect- Other objectives of the program were to document the level of radioactivity in marine organisms from the western Pacific, to provide additional information about the biological distribution of fallout nuclides, and to compare the results of this study with the study following the Redwing test series of 1956. Hardtack fallout that were transported by ocean ing areas, rather than the fallout nuclides trans- ported in the stratosphere. The study following Hardtack supplemented a somewhat similar program that was begun after the Redwing test series in 1956 (Thomas et al., 1958). During the Bikini-Eniwetok phase of Hardtack there were 24 barge, 2 surface, and 2 underwater detonations between May 5 and July 26, 1958. A large portion of the 19 megatons of total fission yield credited to nuclear tests by the United States and the United Kingdom for 1957 and 1958 1959) was produced by these detonations. (Dunhan, Thirty to eighty per cent of the radioactivity produced by the barge and surface detonations could be expected to occur as local fallout (Libby, 1959) and to en- ter the ocean within a day or two after detonation and within a few hundred miles of the test site. Practically all of the radioactivity from the underwater detonations would remain in the ocean. One objective of the study was to determine if local fallout from Hardtack could be detected in ocean waters at distances of 1,000 miles or more from the test site ‘Guam and Palau are 1,200 and 1,950 miles, respectively, west of Eniwetok). SAMPLE COLLECTION, PREPARATION AND COUNTING Samples were collected at approximately three- month intervals beginning in June 1958 and continuing until November 1959, except in the Guif of Siam. There were four collections in the Gulf of Siam and six each at Palau and Guam. Samples in- cluded fish, and plankton. crabs, lobsters, snails, clams, algae, In addition, 72 weekly plankton sam- ples were collected at Palau, site of field headquarters for the George Vanderbilt Foundation. Plankton samples were preserved in ten per cent formalin, but all other samples were sent freshfrozen from the field stations to the laboratory. The samples were held in a freezer until they were processed, at which time they were thawed, dissected, weighed, dried at 95° centigrade, reweighed, ashed at temperatures up to 540° centigrade, and If so, then an estimate could be made of the rate of movement of local fallout by ocean currents, in this instance the North Equatorial Current, since again reweighed prior to counting. 160° 60° al 40° aan - “G es <a A eurran : North Poetic . OB “Kwbsme Et ae TFN IER Rte 8 one, toe 1 ago Figure 1. The major surface current systems in the north Pacific Ocean (after Seckel and Waldron, 1960). 151 DOE ARCHW bo

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