controls, although two hemoglobin samples, 6A and 8A, from the group of 9 individuals exposed to 69 R had high contents of isoleucine.
Table 1.
Frequency of substitution of isoleucine for other amino acids
in human hemoglobin from Marshallese.
Exposure (R)0 controls
175
69
175 in utero
69 in utero
0 offspring
No. of
Substitution
samples
frequency + SEM (x 105)
25
3.09 + 0.33
39
9
&.77 + 0.67
4.45 + 1.16
3
1
5
p value*
0.05>p>0.025
NS
3.26 + 0.64
6.84
5.24 + 0.85
NS
NS
NS
*Probability of significant difference from controls.
Blood samples were obtained from 9 exposed persons in successive years
to determine whether fluctuations in isoleucine content occurred. The data
are shown in Table 2. Fluctuations noted for samples 3R, 24R, 35R, and 71R
could be due to variant clones of erythrocytes arising from individual stem
cells, which appear and disappear from the peripheral circulation. Such fluctuations have not teen observed in specimens from normal laboratory personnel.
Table 2.
Frequency of substitution of isoleucine in samples analyzed in
consecutive years.
Substitution frequency
Exposure (R)
Sample mmber
1974
175
175
175
175
175
69
69
69
69
3R
24R
33R
35R
71R
5A
41A
44A
45A
19.79
13.45
4.74
5.19
8.29
6.98
~~
4.04
3.65
= 34 -
(x 105)
1975
1.76
7.00
2.16
2.17
1.45
6.29
2.56
2.17
3.23
1976
6.69
2.85
3.10