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5
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The radiochemical technique for the separation of the
nuclides by means of ion exchange was adapted from the proce-
dure developed by Swartout et al,
(1944) and Tompkins et al,
(1947).
Preparation of samples
The dried samples were wet ashed with HNOz and H209,
evaporated to dryness and redissolved in 0.2N HCl.
To check
the radiochemical procedures a "control" sample was prepared
in which 0.0Oeu¢ of wi85 was added to a 300-mg portion of dry
Puget Sound fisn and then processed in the same manner as the
test samples.
The samples were filtered and the filtrates were
passed through cation resin columns prepared in the following
manner.
Preparation of the resin column
Dowex 50*,
a sulfonated polystyrene resin of the cationic
type, of mesh size 100 - 200, was washed three times with 6N
HCl,
rinsed three times with distilled water and twice with
dilute HCl.
The resin suspension was placed in a glass column
6 mm in diameter and 100 mm in length containing a glass wool
plug in a constriction at the base of the tube.
Three column
volumes of 0.2N HCl were run through the resin until the eluate
was
clear and the
desitred flow rate was obtained.
* Available from Dow Chemical Co.,
Midland,
Michigan
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