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Combating Detrimental Effects of Radiation
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Project Title:
13.
The Role of Stem Cell and Therapy of the Hemorrhagic Phase in
Radiation Injury
RX-O01-02
Publications:
Matsui, K., Carsten, A. L., and Cronkite,E.P. Effects of storage on CFU of
mouse bone marrow cells,
J. Radiat. Res. 13, 59-70 (1972).
l¥o/dBurlington, H., Cronkite, E, P,, Reincke, U., and Zanjani, E. D.
poietin production in cultures of goat renal glomeruli.
69, No,
12, 3547-50 (1972).
Cronkite, E, P,, and Fliedner, T. M.
Erythro-
Proc. Nat. Acad.
Sci.
J7O Gl,
The radiation syndromes.
Handbuch
der Medizinischen Radiologie, Fritz-Niggli, Editor, Bergmann Verlag, Munchen
(in press).
14.
/7319
Scope:
A)
200 Word Summary:
An effective therapy of the hemopoietic phase of whole body radiation
injury requires histocompatible stem cells for transplantation.
Thus, the
primary objective in this budget activity is to gain more basic knowledge
of the properties of and the relationship between pluripotent cells and
committed stem cells, Marrow aplasia is a major cause of the deaths that
occur following accidental exposure to whole body radiation and during
immunosuppression and chemotherapy of malignant disease,
If a practical
method for transplanting bone marrow were developed, more extensive chemotherapy for immunosuppression and treatment of radiation exposure accidents
as well as malignant disease would be possible. At present there are no
practical methods for stockpiling histocompatible lymphocytic antigen (HL-A)
characterized bone marrow for use in man, although substantial progress has
been made in the development of techniques for preservation of mouse and
..
"
canine bone marrow,
Because of the close relationship of these studies to those reported in
RX-03-02-(b) this work will be reported in RX-03-02-(b) in subsequent years.
15.
Relationship to Other Projects:
Related studies by Lewis and Trobaugh at St. Luke's Hospital, Chicago,
and by Wolf and Trentin at Baylor University, are concerned with the factors
determining the type of spleen colony arising from marrow injections into
we
recipients.
However, they are not examining the same factors as
jr are they examining the total spectrum of colony type and size over
e development period for both endogenous and exogenous colonies as
in this study.
All spleen colony work is, of course, related to the work of Till and
McCulloch, University of Toronto, since they developed the technique and
continue to use it in a variety of studies.
Sensenbrenner and Santos, Johns
(See Continuation Sheet)
—Eb19i849
RX-29