1
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The remainder of the material collected should be preserved in alcohol
or formalin and returned to laboratorics on the mainland for identification.
Types of collection material for sampling
A. Samples for ashing should consist of one specimen from each of 12 species
of fish and 7 plant and invertebrate groups.
The fish groups should include
blennies, cardinal fish, damsel fish, eels, goat fish, groupers,
ligard fish,
parrot fish, siganids, squirrel fish, surgeon fish, and wrasse.
The plant and
invertebrate forms should include algae, clams, crabs, coral, sea cucumbers,
snails ard sponges:
<A determined effort should be made to collect at each station
some specimens of all of the 19 groups. Specimens other than these should be saved
for the general collection.
B,
Trolling and long-line samples should also provide barracuda jacks,
mackeral, runners, sharks and tuna for sampling.
C.
Tissues ashed would be of six types:
(1) entire organism, (2) liver
or digestive gland, (3) viscera or faeces, (4) spleen and or kidneyk (5) muscle
and (6) bone er shell.
Numbers of samples
Ae
The number of ashed samples will be dependent upon thetime and personnel
available, but we feel that at least 1000 ashed samples should be collected in
the field.
B.
The material preserved by freezing would provide an additional 2000 or
more ashed samples for extensive counting and detailed study after the eéturn
to the mainland.
Ce
*
The material preserved by storage in alcohol and formalin would include
representatives of allthe forms used in the field studies.