Natl. Inst. Radiol. Sci. Ann. Rept. (NRIS—10, 197°)
in the table, commonclone cells in the two tissues
%
sf
a3 ff
3
did exist in cases B-17 and Th-3.
In case B-L7, one of the fishermen exposed to
fallout radiation al Bikini in 1954, a clone with a
°
3
4 uf
°
o
x
s
ad
karyotype of 47, XY, mart+ occurred in the bone
marrow with a frequency of about 10%. In the
blood cultures four of the 690 cells (0.58%) showed
the karyotype of this clone.
Jn case ‘Th-3, a man injected with Thorotrast in
roe - G7
e
®
zit
e
<
.
my
e
500
and 70 of the 3,398 cells (2%) in the blood cultures
showed an identical karyotype of 46, XY, Gq-?,
°
e
0
1945, 21 of the 519 cells (4%) in the bone marrow
°
1000
e
1500
8
t(2p-Gpt+)
2000
Minitum VALUES OF NEUTROPHILS
Fig. 10.
Correlation between chromosome aberrations (Cs
cellsjand minimum valucs of neutrophils.
In case RT-1, a cervic cancer with hypoplastic
anemia received radio-therapy, nearly 100% of the
cells in the bone marrow were members of asingle
clone showing a karyotype of 46, XX, t(Bpt+;Cq—) |
and
the derivatives, but none of the 273. cells
snalyzed in the blood cultures showed this karyolype.
Common Clone Cells with Structural Chramosome
Aberrations in Peripheral Lymphocytes and in Bone
Marrow of Irradiated Humans
Takaaki Ishihara, Sei-ichi Kohno
and Toshiyuki Kumatori
The
Ph!
chromosome cine
chronice granulocy Ge
leukemia is known to he present in granulocytic,
erylhrocvlic and mevakaryoeylic cells in bone marrow, but nol in peripheral lymphocytes which are
stimulated to divide in culture responding to PEA,
The evidence has led to an idea that those lymphocyles might be developed from a separate line while
the former three ce!l series are derived from a common stem cell. In mice and rats, on the other hand,
the presenee of multipotent lympho-hematopoictic
stem cells has been suggested.
As a means of approaching the problem on the
derivation of lymphocytes in their relation to bone
marrow cells in humans, three radiation-cx posed individuals who showed presence of distinct clones
with structural chromosome abnormalities in bone
marrow were studied in order lo find out whether
or not clone cells common .to penpheral lymphocytes and bone marroware present.
The results of the chromosome analysis both in
the bone marrow and in the cultured lymphocytes
of the three cases are presented in Table 2. An seen
eel
5010490
The identification of cells with the same radiation-induced markers among the dividing cells in
PHA-stimulated lymphocytes and in bone marrowin
cases B-17 and Th-3 scems to be conclusive evidence
for the presence of a lympho-hematopoictic stem
cell in man yielding both lymphoid and bone marrow descendants.
The present data from irradiated humans clearly
demonstrated that PHA-responsive peripheral lymphocytes are the progeny of the same stem cell for
bone marrow cells, but they do not explain the
reason for the absence tn peripheral lymphocytes of
cells with the Ph! chromosome in chronic granulocytic leukemia or of the clone cells of case RT-1,
which are observed as the majonty of the cells in
bone marrow. This is a serious problem to be solved
in future.
(Unpublished)