2 TEP.
o—_
me
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APPENDIX 3.3.6
"Dr, Giles suggests tcsts on the production of biochoinical
nutants in Neurospora (a) tests could bo made for mutations fron
wild types to biochemical mutants by cxposing microconidin dry,
in tubes or pickots, and mking subsequent crosses to wild type ....
Presumbly, conidia vould be returncd to leb for this bases (b) Tusts
for reversions and reverse mutations at sulectcd loci .....
(c) Yuantitctive data on reverse mutation could be obtcinod with
nicroconidial stocks, however, exposurc would be difficult and
would royuire immediate tcsting,...,
Usual lab facilities for
stcrile work vould be required."
Oak Ridge National Laboratory
Letter of Aloxander Hollaendeor
APPSNDIX 3.3.9.1 - .2- .3 = 4
Prolininary studics have been mde on CF1 mice of the cffoct
of 250 KV radintion on the spleen white pulp/rcd pulp retio and
splenic and thynic weights,
It was found that botwoon 3 and &
days past irradiation, tho weight change could be dircetly quinti~
tated to the amount of radiation reecived,
The chenge in thyric
wcight proved to be the most sonsitive and accurate ‘ncasure of
radiation reecived,
It is proposed thet animals in suitable con-
taincrs to protect frem heat, blast and wind be exposed,
Special
containers sould provide shiclding for ncutron flux,
In addition it is proposed to drop animnis into racinted after oxmlosion in adequcte shiclds at specific tine intorvels,
The clond studies would nocossitate placing inircls in the
drone planes,
Estimite of »ersonnel and Inboratory facilitios is included,
L, fA. Seientific Laboratory
Dr. Rebort Carter, ct al
APPS] DIAGS
3.3.6
3.3.9.1
- 10 -
GS i ana
-.2
-.3
“4