az port wt 7 a _APPENDIX 3.3.2 "Drosophila should be exposed and genetic effocts studics. These studios should be done immediately after oxposure wees Because of the differenca in relative efficiency of neutrons and gamma rays for the production of (dominant lethals and recessive lethals) it should be possible to find the proportion of fast neutrons to gamma rays at the point of exposure. University of Chicago Froposol of Dr. Franklin C. McLean The insect species that the NME project, M-11, proposes to use are not known at this time. Capt. Draveger's group at the Neval Medical Research Institute have devoted much thought to, and have had experience with conteincrs which oro suitable for exposure of small material of this sort, <A drawing of the containers used in previous atomic tests is attached. Capt. Dracger will comment on the problems peculiar to this sort of study. APPENDIX 3.3.3, "Dr. Conger suggests tests on tho offect of bomb irradintion on tradescantia chromosomes, This could demonstrate whethur biological cffects arc predominantly from gamma rays, or from hoavy particles (neutrons). Tho approximato radistion lovels to bo tested are from 20 - €00 r xeray oquivalont effoct..... Plants would be placed at various distances,.... and collected within 2224 hoursS..e. Simultancous exposures would be made of control plrnts to a burst of x-rays, or gamma rays, at a rate in excess of 200 r/ minute... Noed facilitivs for koening plants in geod condition until they could bo pleced (for expourc).... We would like to study as many as several hundred plants in pots ..... Infloroscences could be put in viels in differont locations .... (these) would have to bo available for analysis a few hours after the blast, Equipvment required: Small laboratory space, usual glassware for microscopic study, microscope. Oak Ridge Hational Laboratory Letter of Alexander Hollaender (Continued on next page) APPE™DIXES : Beaded 3.3.5 y=) ey,

Select target paragraph3