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eae
pang
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gluccse uptake by niuscle of the forearm of mag: lease (fom adipose tissuc, a tact demonstrated‘i?
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bt
ROLI OE PRE EVERY ACIDS IN FOREARM METABOLISM
IN MAN, QUANTITATED HY USE OF INSULIN ¢
Blood yohuns and ite regulation. Ann. Kev.
Physiol. 1957, 19, 231.
Va, Ailes, J. Hy Peug. M. T., Chen, K.P. Huang, TF,
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LT.
8
foutnal Cink
Inve sisgutiom
(!
bath in wea by Gordon (4), Kates and gssociates 3
(§), and Bierman, Schwartz, avd Dolg (6), an. i
in mitra (7, 8). There ig, bawever, qonilleting |:
eyidenog ov whicther or nut insulis influences FRAG:
uptake by periphera] tissueg, Is excised adipoag' ‘sr
dggye, in the presence of both glucose end dass
lip, FFA igcorporation ia increaged (7). But. |
thare ip no quch evidence from experiments fa ,
wipe. Indeed, it is unproven that there is euby':Ar
wansial FFA uptake by adipose tissue in vive une i. der any circumstances. Bragdon and Gordon (9)"°
found only 1 w 2 per cent of injected palmitic :
Friedberg, Klein, Trout, Hogdonatf, and Kostes aghj-1-C'* in adipose tissue, a figure not ipereneed
(3), that the human forearm does reniove FFA ' by prior adwiinistration of glucise, which stimu-,
i
jfrom artecial blood, and the possible factors ree
‘sponsible for our failure to demonstrate this by
simple measurement of A — DV concentration
differences have been treated in detail elsewhere
(2). bn brief, while FIFA is being extracted from
plasma by forearin inticle, adipese tisste, fntithalely surrounding iiiade, adds fatty acid to ve-
nous lasing, The uct A - UV FEA difference
in determined by the relative rates of these two
pracessts.
The validity of this hypothesis coukl be tested
if there were an agent that inhabited release of
FFA from adipuse tissuc withuut also affecting
FEA uptake by peripheral tissues. Insulin may
be such an agent,
* Aided tn part by Comteact None -248(34) (NR LUE
24h) between the Ore of Naval Keaearsh, Departincot
of the Navy, and The Juluis hopkins Universiny, Baleiwore; by a grant-in-aid fro the Muscular Dystropiy
Associations of America, bu., and by US. Pubhe
Health Service Kesearcls (stants A-750) and A-5524 and
Graduate Training Grant 2A-$252, National buatitute of
Arthritis and Metatohc Diseases, Hethesde, Md Reproduction in whale uf in part is poruaitted hy the authors
fur any purpose of the United States Goverment; pers
Inisslun of publisher cequired,
{ During tenure of a Wellemne Research Travel Grant,
lates insulin secretion. It haw been demonstrated by Spitzer and Huheuleiiner in the dug (10) and
by Scow, Kobert, and Cheick (11) in isolated
rat purametrial adipose tissue phat, while det prow >
Tpction af FFA by adipose tiesue was inhibited hy.’
tndulin, stgutheant vet wet by adipusge tleue
waa nut produced. With rediact to a possible ef
feat of insulin on FEA uptake iy auacle, experi. ineats capable: of testing this crucially 1 ove have
2
uot been done. Absence of any such action, howver, is suggested by the fact that turnover rates
uf FFA, measured by use of isutopes in the dog,
ace nut changed by imsulin (0. 12). Fritz (13)
showed that oxidation of palace acid-1-C# by
excised rat diaphragur was not alicred by insulin,
Thus, while it js not established that insulin may
nut influence FFA uptake by peripheral tissues, _
most uf the available dats suggest that it does - »
not do su in the intact anual. In the light of
Previous studies showing that the human foreann ©
i> remarkably sensitive ta local intra-arterial inr
fusion of sanal) concentrations of insulin (14), a.
tcchnique was available to establish the effecta of
insulin, injected intra-artcrially at a final concen-'
tration pf several hundred miccounite per milli-
ay)
|