leve1.°
- trophic
Tuna
Euthynnus
and
mackerel
nuda
Gymnosarda
and
Jacks
crustacea.
benthonic
on
primarily
Grammatorcynus
the. fourth
in
are
snappers
Thunnus
albacares,
billineatus
are
(bonito),
affinis
feeding
is a bottom dweller
Another snapper Letherinus kallopterus (pigfish)
and
large,
rapid-swimming carnivores feeding on small fish and any other prey of proper
They represent species of the fifth trophic leve1.®
size.
SAMPLE PROCESSING AND ANALYSIS
The
fish
samples
partially thawed.
fish
was
from
location
The total weight,
determined.
Each
(cranial and thoracic,
skin and
each
scales
fish
numerically
standard or fork length,
was
dissected
vertebrae and ribs,
(fins
were
discarded),
into
counted
and
and sex of each
muscle
tissue,
bone
and pelvic and pectoral girdle),
stomach
contents,
liver,
and
remaining
viscera that included large and small intestine with contents,
stomach wall,
spleen,
organ
kidney,
and
mesenteries.
Each
separate
species from the same catch was pooled.
but
not
analyzed.
Our
experience
be
little
possible
academic
dissected.
viscera
Adductor
that
Clams
were
muscles,
included
from
1978 showed
plus
gonad,
measured
siphon,
stomach,
because
(total
kidney,
intestine
from smaller clams such as the Tridacna crocea from specific
for
analysis.
individuals.
For
the
larger
species,
After the wet weight was determined,
sample was dried in ovens at
fish
gills
were
of
the
length),
and
and
remaining
and
contents,
crystalline style, heart, and nervous system were removed for analysis.
pooled
the
from the
the
their analysis
weighed,
mantle
gills,
to
of
The gills are not eaten and there could
information gained
contamination.
and
Gills were separated
prior
sometimes contaminated with sediment.
tissue
analysis
Parts
locations were
was
made
on
each fish and clam tissue
90°C to constant dry weight
and
dry ashed
in
muffle furnaces at 450°C for approximately 72 h.
Samples were transferred to aluminum containers,
gamma
spectrometry.
Gamma-spectrometry
sealed, and analyzed by
measurements
were
made
on
all
separated samples at LLNL using a variety of Ge (Li)-diode detector systems.
Counting times were usually 1000 min or longer for each sample.
A
general-purpose
reduction
long-lived
of
all
nuclear
computer
generated
explosion
program,
spectra.
products,
GAMANAL,
The
program
activation
was
used
searches
products,
for
a
and
the
library
data
of
naturally