-10- The transition elements, nickel, iron and zinc,were determined described by Sandell (1959). rated. manganese, cobalt, colorimetrically by The elements were initially sepa- Kraus and Moore (1953) have shown that the chloride complexes of the transition elements, are adsorbed onto a (Dowex 1) HCl. methods by the selective elution of their chloride complexes from an anion exchange resin. zinc, copper, strongly basic nickel through anion exchange resin and are selectively eluted at different molarities of Following the same principle, Joyner and Chakravarti (1960) have suggested techniques which were applied to these samples. Light transmission of the solutions containing each of the separated icns was compared against sets of standards with a Bausch and Lomb "Spectronic 20" adjusted to specific wave lengths. Protein nitrogen was determined by the Kjeldahl method. Fat was determined by a modification of the Johnson method (Winton and Winton, 1945). ‘Methylene chloride was the extracting solvent. Ash content was determined as the nitrate form by drying an aliquot of the ashed sample to constant weight. The moisture content of the sample was calculated from the wet weight to dry weight ratios. The total carbohydrate and like substances were estimated by subtracting the moisture, fat, protein and ash from the total