-5tube.
Three-colum volumes of 0.1 N HCl were run through the
resin until the eluate was clear and the desired flow rate was
obtained.
The quantity and mesh size of the resin bed for the
experiments differed slightly as indicated in Table 1.
3.
Elution procedure
The sample was allowed to pass through the column at a rate
of 0,2-0.5 ml per minute and then the column was washed with 2060 ml of dilute acid of the same normality as that in which the
sample was dissolved,
The flow rate was controlled by adjusting
the height of a separatory funnel containing the appropriate elut-
ing agent.
The above fractions contained those ions not adsorbed
to the resin and were designated as the anion fractions.
The cat-
ions adsorbed to the resin bed were removed progressively by passing through the column the required volumes of 0.5 per cent oxalic
acid, and of 5 per cent citric acid adjusted to pH of 3.5, 4.1,
4.6, 5.1, 6.6 and 6.1 with NH,OH.
In some of the experiments a
mixture of 0.15N NHC1 and 0.1N HCl was used to remove cst37 ,
Subsequent experiments with spikes have shown the NH,C1-0.1N HCl
step to be ineffective and its use has been discontinued.
A summary of the elution schedule for each experiment is
given in Appendix Table 1.
The eluates were collected in cali-
brated test tubes in fractions of 2-20 ml, either by hand or by
fraction collector, and aliquots of these were used for radio-
assay.
The resin bed was removed from the column at the termina-
tion of the elution and was ignited for radioassay.
In some of the experiments certain fractions were passed
through a second resin column for further separation of the nuclides.