I. The basic program of the Applied Fisheries Laborat the effect of X+rays upon aquatic organisms, was the major concern of the staff. for er u ay Oye Bebe *pee 1 By: Date: xSections I and II | hotAsnorzes for Punic Feteuse ‘ By: Date: During December the balance of the eggs produced from the adult salmon returning to the Samish River, that in turn had been produced by adult chinook salmon exposed to 100 r of X-ray prior to spawning, or that were used as "controls", were transferred to the lboratory at the University of Washington. The majority of the eggs have now hatched and are in the yolk sac stage. The 38 irradiated- and control-stock chinookcsalmon adults retaken in 1947, produced 15 egg lots, the spawn from 13 of the females was apparently}e mature and normal while the spawn from two females (Lot 13 and Lot 14) appeared to be immature or "preen', | Further investigation into the inferior nature of the eggs in Lot 13 (100 r - 100 r X-rayed stock) with Lot 14 (control stock) revealed that Lot 13 was from a "green't or slightly immature female, and consisted of 6059 poor eggs, deformed, including many of a pearshape. hatchery procedure Lot 13 wuld have been discarded. In ordinary Egg Lot 14 was also from a "green" female resulting in a relatively small percentage of the eggs flowing out into the bucket when the female was opened, but the 1386 eges that were taken seemed at the time to be normal and would have been retained in ordinary hatchery procedure. When Lot 13 is for this reason excluded from Section numbers refer to the Project Chronology Chart, revised Jamary 9, 1947.

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