%
.
'
the NTS, are expesed te high energy x-irradiatien at six expesure peints
straddling a previeusly determined 1509/3 ef 500 rep. Animals are
2th under the 2 MEV Van der Graaf
exposed at the National Institutes of
unit at the time the animals are expesed to weapon irradiatien.
Sub-project .2.3. Nineteen hundred and twenty Walter Reed - BAGG
strain ef mice. Only female mice are exposed, weighing appreximately 25.0
grams at expesure. The axes for animal placement parallel that fer the
' swine, and, as such, en ene axis the total dose ef irradiatiqn consists ef
at least BOF neutren flux (south axis).
This is feasible on the basis ef
weapen geemetry and without sophisticated shielding other than te avoid the
thermal flux and te prevent blast injury. The second axis fer animal place-
ment is such that the total dose of irradiation delivered censists of 80%
gamma with 20% neutren flux centamination (west axis). Animals are exposed
in aluminum and lead hemispheres (ITR-1167, Operation TEAPOT-Classified) in
such a fashion that there are fifteen aliminum stations on each axis and en
the south axis there are fifteen lead hemispheres, Dispesitien is such that
the statiens cover a dose range ef 300 to 1000 rem, with forty mice at each
point.
As s00n as pessible after exposure the mice are recovered and returned
to the animal facility. Twenty mice frem each peint will be simply ebserved,
with death as an endpoint, for thirty days (LDso/
).
Ten mice fren each
point are te be sacrificed at. 9 hours, and ten Vice sacrificed serially
ever the 30 day ebservatien period. At sacrifice the fellewing are carried
eut:
1.
net spleen weight
2.
thymus weight
3.
small intestine weight
4.
total peripheral white cell count
5.
differential white cell count
6.
gress body weight
7.
‘tetal bene marrew count ef the right femur
8.
and appropriate histological sectiens are fixed as demanded.
Control mice for serial sacrifice are given identical field handling
and exposure cenditiens, but without actual radiatien exposure.
At Walter ee
Reed Army Institute ef Research, after return from NTS, mice ef the same
strain, age, weight, etc. are to be subjected te an We
analysis of parameters identical to the field study.
estimates even more reliable.
Sub-preject 4.2.4.
study with
| 4.4
should make RBE
See Program 2 experimental plan.
nae
°°