ert ee re ee AECD-3446 -60- 4 selected at the direction of the island leader, It came from an area frequenteq permit relatively larger constantly by the inhabitants whose habits are such as to amounts of human wastes to become available to the plants than was the case for “aeet coconuts grown in plantations, or on islands which are not permanently inhabited, a 2 In this manner it is possible that the control material from Likiep is relatively richer than the majorityof coconut samples in certain ash constituents such ag FE i potassium and hence K It was considered desirable to include some plant materials which were gcown in soils commonly used for agricultural crops, and to be still more cautions,4 ; to include some seeds which are known to have been confined in stoppered glass bottles since before the beginning of the atomic age. i.e,, about 1938 at the State College of Washington, Clover and parsley leaves and spinach and sweet clover seeds were selected for this comparison. The coconut tissues were selected and prepared for ashing at the State — College of Washington and were ashed, weighed, and counted at the University { ee of Washington (under the same counting conditions as were used for the aquatic . forms). The procedure for preparation of coconut samples for radioactivity assay was as follows: (1) Dry material to constant weight at 85°C. (2) Cool in dessicator. (3) Weigh out a representative sample of | gm. (4) Place in a porcelain crucible of known weight in a cold muffle. (5) Ash to constant ash weight at 500°C to 550°C. (6) Cool in a dessicator. (71) Weigh to get ash weight (usually around 15 mg/gm dry matter). (8) (8) Transfer solid ash to a 1-1/2" stainless steel counting plate. Dissolve remaining ash in a minimum of 1:9HC1. (1 pt. conc. HC1:0 pts. H,0) transfer to the ss. plate. Evaporate the HCl, Repeat the washing twice. (10) Spread the ash uniformly on theplate. (11) Dry under a heat lamp, removing all HCl. (12) Count. « PASTE Seres “ TAT, Bees tak sO, UNIV. us