AECD-3446 -18- and Runit - were along the northeastern portion of the atoll near th# three test sites of Engebi, Aomon-Biijiri, and Runit. The other three - Japtan, Igurin, and Rigili - were seven to fourteen miles from the test s3t©&- Plankton and water samples were collected while at anchorage off Eniw#tok, a eo Engebi, Aomon-Biijiri, and Runit Islands, Additional algae and invertebrate samples were collected by dredging off Eberiru Island. Fish traps were set off Aomon-Biijiri Island. (For Eniwetok collecting stations see Figure 2), Eniwetok collections were made between August 8 and 15, 1949. At Likiep Atoll major collections were made both on Lado Island and on the western end of Likiep Island, Plankton and water samples were taken at anchorage off Likiep Island. The Likiep collections were made during the period of August 19 to 22, 1949, PRESERVATION OF SPECIMENS A change in procedure introduced in 1949 was the ashing of all samples at the University of Washington Applied Fisheries Laboratory rather than at a temporary laboratory established aboard ship. The rea~ cE sons for the change were for the purpose of increasing the accuracy of weighing samples and of controlling the ashing procedures, In order that e wt : . - &§ the samples be as similar as possible to the fresh samples of former years, specimensfor radio assay were frozen. From experimental work per~ formed in 1948, it was known that samples prepared from frozen specimens : ie were comparable to samples prepared from freshly killed tissue (see UWFL 19, pp. 10-12), Therefore, in 1949, specimens of fish, algae, in- 3 vertebrates, and vertebrates that were to be ashed for counting were cooled oa withice while still in the field, then were moved to a deep-freoze box n. §. aboard the LSIL1001 for storage. During the air flight from Kwajeleinto Seattle the specimens to be used for ashing were stored with dry ice in an insulated container, The specimens arrived at the University of Washing- ° in a deep~ ton laboratory in a frozen condition and were immediately stored " An freeze unit where they remained until time for dissection and ashing. v effort was made to freeze the specimens on the spot at the time of collection, i dioxide, but this using a fire extinguisher containing four pounds of carbon & an . ne > rH) ERIVERS cee ARCHIVES , unty. OF 4 YY 1 ye want ie Be. Ey OT