to praise ayperpiacia in sae catients. /lthourh we must admit that tac iene detine chyroid stinalator (LATS) is the mechanica we anp not covincmed which [Reaves the thyrGid 2A Geaves' «ajsease, it is demenstmbie in at least half of thebe puticonts moter: Seoatnent. we have the LAGS assay cweithad firmly establishes? infcur Lakzatme-“Se mltheurch, 13 Beate Agova, Wwe had no of portunity to obtain rediated h aa tht tissue of “raves! Udiocas? curing tne past year and to camry aut cur us thyrsid) Sinsue with sootlal at lenticn to the occurrence of bizarre for patients, whom the epoertundty does arice we are Prep wv) to see wheth ap he he “NG Tebova Taw any mbationchip wy the Dbsieres nuclear ferns] huaweiinveostids. ARQ LCGGF CUOACHOSOMETD TH CLRCULATTIING LEUKOC NPN TOLLOWONS THERAPZUTIC boces or llr Bee in sea thea rresermme $ sand in sco TES teak subte strovatieng fnowhis country cn chresacwl ufccallocs ia cipeuse tains leukeeytes following lerye deses of Wwhre mela: $ ter this contract. 451T fer carcinoma of fhe thyreid These observations were mide ‘ci!lowing a very 292 done of +423. Mot only wag there an acute and markerl rise in the inci anwralias in chremésoms of the circulating leukecytes, but residual eff : form of ancsalies fron previocs massive dosea of 481% nad persistedsSiz spears. Since thet tim, Vac have attesrpted to detect chromosonal anmelics treated with the usual therazeritic doses (Five to fifteun mi lLicuries) for G1raves! disease. Some publications nave appeared describing changgs doses of 4447 of this rane, bat from our gradually accumulated experi 3 caused by has bean wore ques tien of the validity of cenehstens based on simple observations. we .ive felt that it was necessary to make observaticnslors a love series af blocd Samples, with multiple culturms fron each, and a largelmrnber =f peparetions fron each cultur2, along with extensive pretreatment con to muha sucn cbservaticns valid. bavid Satcher, an M. D., Ph. D. candi SONNE under or. leil Macintyre (with when we published the first work), hes Fecn stvfans this probien of ‘chresesonal aberrations in sere of the 1511 treated pa were studying in detail. Althouzh he has studied relatively few cases them in great detail as implied above. this thesis entitled, wane Effe Aeradiaticn cn the Chrosesomes of Feripheral Blocd Leu?kocytes™ > has j end his dezrees are being awarded. He has studied the effects of dose from 6 to 19.7 me. An increase in the frequency of chromosomal aberra which reached a peak at 24 hcurs and fell to almost control levels by treatment. Se has not found a relationship between the dose of lslri increase in frequency of chrescsane aberraticns in time. Some patientg h=ve = greater mspcnse than others without a readily available explenatio. from the ccse-respense curves for aberrations that only leukeeytes al streaa at the tine of 1-4] therapy were involved and that precursor ce Significantiy canared. He has very carefully analyzed a suspected ce of exrcr st may have contributed <9 misinterpretations of data produced by scme otiiers. T= queséscn a3 lcogicall, raised «hether the i3ly Circulating in the blood sample 15 taken and <ransferred to the culture contributes to the ci while these cells are in culiime. To testthis question, a goodly a was cbtaincd from the patient tefore the 1slr was administered. This serun was then substituted fer the patient's radicactive serua «hen tomes were preparcd subsequent to giving the patient 131;, Conversely the hot” to pretreatment control cultures. It was shown that residual 4311 culture continued to damage chromosanes while the culture was in p Le Dwele: . aR “ oem, + oe, 2 nob ors Los cooa a ne i oo * ~ ON ae Paid or