Attys C4 Proposul* Numbor Oel THE PROGRAM OF TESTS APPROVED BY a AD HOC COMMITTEE Project 1: Animal Colony Responsible Agency: National Military Establishment (NMRI) Objectives To provide an adequate number of micc, dogs and pigs. “These animals would be acclimatized to the total environment, Their LD5q for 250 KV x-ray would be detcrmined aftor acclimatization, It is planned to provide 5,000 - 10,000 mico of the CF=-1 strain; 500 dogs, preferably tho Rochester bongle strain; ond 500 pigs of the U. S. Department of Agricul- ture “hairless” strain. Procedure: If approved, 2 Neval party will ostablish the colony at once to permit approximatcly 18 months of brecding. A veterinarian, indoctrinated in genetics, will accompany the party. Now animals will not be introduccd into the colony later than ono year before the expected date of the tests. Sufficiont animnls will be bred to provide adequate controls, A roserve colony of equal size will be developed at the NMRI, in case of accidents, After the tests, representatives of the species, and survivors of tho tests will be returned to NMRI for further study. Project 2: 342-161 Study of Acute Radiction Injury Responsible Ageney: Objoctive: National Military Establishment To expose enough animals in enough positions to obtain valid statistical data for a study of acute lethality, vs distance «nd dose: LD5q vs distance and dose; vs distance «and dose. and survival Procedure: Micé, dogs and pigs will be placed in containers suitably shiclded from heat and blast, These will be placed in the water snd on land at predetermined distances from zero peint; and are to be located so that they may be retricvod promptly. Animals will then be observed, sacrificed serinlly,cte. Becel.t Responsible agency: «argonne National Laboratory Objective; To attempt to separate the effects of prompt nuclosr gamma radiaticn, neutron radiction, and gamma radintion from the fireball, Procedure; Mice will bs plesed in heavily shicided containers with collimiting slits directud toward the detonation point, and toward the estimated location cf the fireball, Acute lethality, LDsgg and survival will be s tudicd. Resnonsible Agency: National Ifilitary Establishment Objcctive: To obtain animal tissucs at the earliest possible time after cxposurce, and serially thereafter,for detailed histologic and hematologic study. There is a lack of information from Japan and Bikini on this carly period, . Procedure: «Animals exposed as in 3,2.lelwill be retrieved at onec,. somo will be sacrificed at once, others serially at aporopriate intervals thereafter, The tissucs would be fixcd and preserved for study in the Naval Medicnl Rescarch Institute, the Army Medical Center, and the army Institute of Pathology. Blood studies would be made in the Island Laboratory. Beleded Sele tet Responsible agency: National Military Establishment Objective: To obtain animal tissucs at the carlicst possible time after irradiation, and serially thereafter for histochemical studivs, Such studies are boing performed on tissues irradiated by conventional means, but none have been done on tissucs exposed to atom bomb radiation. Procedure: Tissues as obtained in 3,2,1.1 snd 3.2.4.1 will be guick frozen at -55°F, and stored and transported in this condition to the .irmy Medical Centor for histochemical study. Responsible wigency: pee National Military Esteblishment To obtnin animal tissues at the carliest possible time after irradiation, and serially thereafter for studics of tissue enzyme systems, ouch studies have been performed #Refers to Agenda with x-radiction; none with the intense atom bomb radiation, Yee