Health Effects of Chemical Toxicants and Effluents Determination of Trace Metals in Human Tissues and Project Title: Their Influence on Certain Diseases RX-01-04 15. Relationship to Other Projects: (Cont'd.) and the influence of metals upon pulmonary function, He also will contribute substantially in the obtaining of human specimens. The x-ray fluorescence studies of Dr. M. Hillman, BNL (DAS) is essential, related work, There are many investigators working on trace metals, particularly cadmium. In no instance, however, does anyone else have the unique strain of rats that is used at Brookhaven to study the pathogenesis of experimental hypertension, hypercholesterolemia, and atherosclerosis, These rats provide an unusually sensitive tool with which to study additive factors in diseases of multifactorial causation like hypertension and atherosclerosis, 16, Technical Progress in FY 1973: Project to be initiated in FY 1975. 17. Expected Results in FY 1974: Project to be initiated in FY 1975. 18. Expected Results in FY 1975: It is anticipated chat firm design for construction of automated exposure of samples in RARAF, its fabrication and operation would be completed. Neutron activation analysis and chemical techniques would be used for initial study of human tissues, Methods for obtaining fetal biopsy and autopsy material would be established. . The baseline levels of vanadium and cadmium in the four strains of rats mentioned above will be determined, .These values will be correlated with cholesterol and triglyceride determinations as well as blood pressures, effects on these parameters of small quantities of vanadium and cadmium The added co the diec will be determined, At the same time, initial studies will begin on measurements of these two metals in patients with hypertension, A correlation with cholesterol and triglyceride levels will be made, 19, 2 ription and Explanation of Major Materials, Equipment and Subcontract FY 1975 Capital Equipment: Six each flush type rat cage racks would be procured and installed in an animal Laboratory for the initial studies ($13,000). An autoanalyzer is necessary to perform the cholesterol and triglyceride determinations thac could not be processed on existing equipment ($18,000). (See Continuation Sheet) ; af /! fe C $179259 RX -98