Exposure to External and Internal Radiation Project Title: 17. Medical Studies of Marshall Islanders and Study of Radiation Effects on Intracellular Mechanisms Expected Results in FY 1974: (Cont'd.) RX-01-01-(a) bicchemical and histo-chemical studies; (3) metabolic studies of separated purified organelle fractions by direct stimulation of separated organelles with the mitogen; (4) identification of the nuclear status of the mitogen and its complexes by chromatographic techniques. in vivo studies on the mitogenic action of the purified molecules on the lymphatic system will be initiated in rabbits, The development of a specific antibody against the mitogen will be complexed with the tagged mitogen to study possible membrane localization and the effects of mitogenic action. 18. Expected Results in FY 1975: Careful screening of the Marshallese populations for development of malignancy, particularly of the blood and thyroid gland and the development of other degenerative diseases will be continued on a long-term basis, Upon return of the Bikini and Eniwetok people to their home islands, it is antici- pated that whole-body gamma spectrographic analyses will be carried out on these island groups as well as on the Rongelap people again. Upon completion of the year that Dr. Knudsen has agreed to stay on the islands, it is hoped to obtain the services of another physician who will carry out these important supplementary health service functions. The services of such a physician are extremely valuable in putting the thyroid treatment program on a firm basis and in documenting health statistics on the Marshallese. Studies will continue on localization and mechanism of action of the purified mitogen, induction of cellular transformation, and cell division in normal and leukemic lymphocytes. The in vivo use of the purified mitogens in animal studies will be of great interest for possible therapeutic considerations in view of the greatly reduced antigenicity of the purified mitogen. It is hoped that the basic defects in chronic lymphocytic leukemia and other disorders of immunologic competence will become apparent in the ensuing years. 19. Description and Explanation of Major Materials, Equipment,and Subcontract Items: FY 1975 Capital Equipment: Recellaneous equipment for conducting the field surveys and for process- M@Pimens collected for laboratory analyses at BNL is estimated at $10,000. A quartér share ($25,000) of the automatic autoradiographic grain counter and cyto-analyzer described in Section 19 of RX-01-03-(d) will be charged to this activity because of use of the equipment in cell studies. (See Continuation Sheet) b1T9U1b8 RX-8