Molecular and Cellular Radiobiology
Project Title:
14,
Scope:
Effects of Radiation and Chemicals on Control of
Hemopoiesis
RX-03-02- (b)
(Cont'd.)
Studies in man are aimed at é@lucidation of the defect
population of cells compared to normal cell proliferation.
in the leukemic
Leukemia
is studied by a combination of techniques: measurement of cell kinetics
following administration of tritiated thymidine; serial sampling of the
bone marrow and peripheral blood; culture of leukemic cells in diffusion
chambers implanted into the mouse complemented by in vitro culture; and
measurement of DNA content (when facilities are acquired).
The enzyme
profile and the karyotopes of chronic granulocytic Leukemia cells are
studied before and after culture of cells in diffusion chambers.
Radiobiological
studies are concerned with determination of the De
of the human HSC required for radiation therapy and space flight and the
relative biological effectiveness
the CFUs,.
15,
(RBE) of various neutron energie® on.
‘
Relationship to Other Projects:
The studies on lymphopoiesis reported in RX-01-03-(d) are a closely
related research program.
Elsewhere,
related studies include those of Lewis, Trobaugh, Fried and
Knospe, St. Luke's Hospital, University of Illinois, on factors in the
°
bone marrow and spleen which support hematopoiesis, and the hemopoietic
’
inductive micro-environment in the spleen and bone marrow,
Till and
McCulloch, Department of Biophysics, University of Toronto, the initiators
of the splenic colony work, are concerned with short- and long-term feedback loops regulating growth, differentiation of the stem cell, the effects
of drugs on the stem cell and the relationship of the stem cell to leukemia.
Stohlman and Quesenberry, St.
apply splenic colony formation,
Elizabeth's Hospital, Brighton, Massachusetts
erythropoietin administration, in vitro
bone marrow culture, and more recently the diffusion chamber technique
learned at BNL, to study growth kinetics and factors related to erythropoiesis and granulopoiesis.
Benestad, Breivik and Boyum, Oslo, Norway,
the developers of the diffusion chamber technique for study of hematopotesis,
continue its application to ascertain the nature of the factors which
influence stem cell proliferation and differentiation.
ies inson, University of Colorado Medical Center, uses the in vitro
of murine and human blood bone marrow cells in patients with various
pscrasias and in studying the regulatory factors concerned with
granulocytopoiesis.,
Rothstein, Athens, and Cartwright, University of Utah, apply the in vitro
culture of bone marrow and blood cells along with a modification of themillipore diffusion chambers to study stem cell proliferation, differentiation, and
factors regulating granulocytopoiesis,
(See Continuation Sheet)
1119288
RX- 228