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Fliedner, Cronkite, Bond and Andrews, Mitotic Activity and Cytology
In Pappenheim stained bone marrow smears it is sometimes difficult to
distinguish between normal and abnormal mitoses, especially those in prophase
and metaphase. In the first marrow aspirates after 12 and 44 hours, however. a
relative increase of anaphases and telophases was noted. In these smears 60 to
90°, of all mitotic figures showed abnormalities. Atypical mitoses were seen in
both the myeloid and erythroid series.
Chromosomal stickiness was recognized in metaphases and the later stages of
cell division. In some metaphases, 2 or 3 chromosomesstick together forming a
B
C
nee
Fig. 2. A: Late metaphase with chromosomalstickiness 12 hours after the accident: B: Myclo-
cyte division, +4 hours after accidental exposure with a chromosome fragment. C: Normoblast division, 12 hours after exposure with a chromosome fragment. D: Myclocyte division.
12 hours after exposure with two lost chromosomes. Note binucleated normoblasts and in one
of them a cytoplasmic chromatin clump. E: Chromatin clump in the cytoplasm of an abnormal large oxyphilic normoblast, 12 hours after exposure.
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