Phase I will use three (cor more) goats and Phase II will use four cows as experimental animals, Single oral or citrate-buffered intravenous nuclide doses will be given to each animal. Both phases will be conducted at the Nevada Test Site Farm and the collection procedures will be similar, Milk, blood, urine and fecal samples will be taken for analysis over a six day period after dosing. Pretreatment samples, to include those used as counting standards, will also be collected for analysis, Approximately seven days after dosing, the animals will be sacrificed, at which time tissue samples will be taken to establish the distribution pattern for americium-241. REFERENCES Ballou, J. E. "Effects of Age and Mode of Ingestion on Absorp- Proe. Soc. Exp. Biol. Med. 98:726-727. Barth, J. 1977. “Application of the Artificial Rumen and Simulated Bovine Gastrointestinal Fluids Procedure in the Study of the Bioavail- ability of Transuranics." Gamma counting ie considered the most feasible analytical technique for use in conjunction with these experiments, Americium concentrations in the sample material will therefore be determined by counting the 60 keV gamma-ray of americium~241. Selected samples will also be prepared for radiochemical analyses, II, 1958. tion of Plutonium." This publication. Barth, J., and A. A. Mullen. 1974. "In Vitro Plutonium Studies Using the Artificial Rumen and Simulated Abomasal and Intestinal Fluids." In: Dynamites of Plutoniwn in Desert Environments. P. B. Dunaway and M. G. White (Eds.). USAEC-NVO-142. pp. 143-150. Finkel, M. P. 1947. "Transmission of Radio~Strontium and Plutonium From Mother to Offspring in Laboratory Animals." Phystological Zoology. 20:405-421. Biological Transfer of Actinides via In Vivo Labeled Food Finkel, M. P., and W. E. Kisfeleski. While there are currently no specific plans to continue studies on the gastrointestinal uptake of nuclides from in vivo labeled food products, the authors believe that such studies would be of considerable benefit. Furthermore, studies which also include pigs and chickens should continue to be part of the overall program because of the significant contribution these species make to food production, 1976. "Plutonium Incorporation Through Ingestion by Young Animals." In; Health Effects of Plutonium and Radiun. W. $. S. Jee (Ed.). J. W. Press. Salt Lake City. pp. 57-69, Forth, W., and W. Rummel. Reviews, 53:724-792. 1973. "Iron Absorption." Physiological Katz, J., H. A. Kornberg, and H. M. Parker. 1955. "Absorption of Plutonium Fed Chronically to Rats." Am. J. Roentgenol. 73:303-308. McClellan, R. 0., H. W. Casey, and L. K. Bustad. Some Transuranic Elements to Milk." Health Phys. 1962. "Transfer of 8:689-694. Miller, C. L., J. G. Payne, Jr., E. W. Bretthauer, and A. A. Moghissi. 1972. “Transfer of Plutonium From Milk Into Cheese." (22:563-565. 10. Health Phys. Miller, W. J. 1975. "New Concepts and Developments in Metabolism and Homeostasis of Inorganic Elements in Dairy Cattle: A Review." J. Datry Set. 58:1549-1560. ll. Mullen, A. A., 5. R. Lloyd, and R. E. Mosley. 1976. "Distribution of Ingested Transuranium Nuclides in Chickens and Subsequent Transport to Eggs." In: Transuranium Nuclides in the Environment. International Atomic Energy Agency. pp. 423-433. 12, Mullen, A. A., and R. E. Mosley. 1976. "Availability, Uptake and Translocation of Plutonium Within Biological Systema: A Review." EPA-600/3-76-043. 447