\ bn. OGCUMENT SCURCE oo University of California at Berkeiey The Bancroft Library/The Universay Archives. Berkelay CA REC ORCS SERIES TITLE Kenan th Gorden Scott Pars BANCROFTUAAC iD NO. Boca} CARTON NO. —_— ! COPY FOLDER NAME RB: acu bh ‘454% NOTES p / (FQ 3 £413 FOUND BY/DATE FOUND ore He lobe LO LILI SGY 401900 BEST COPY AVAILABLE y Reprinted from THE Jovrnat of Cotxicar [xvesticatiox, Vol. 38 No. 7, pp. 1065-1077, July, 1959 Printed in U. 8. A. RED CELL, PLASMA AND BLOOD VOLUME IN HEALTHY MEN MEASURED BY RADIOCHROMIUM (Cr*'!) CELL TAGGING AND HEMATOCRIT: INFLUENCE OF AGE, SOMATOTYPE AND HABITS OF --.- PHYSICAL ACTIVITY ON THE VARIANCE AFTER REGRESSION OF VOLUMES TO HEIGHT AND WEIGHT COMBINED * By R. WENNESLAND,+t ELLEN BROWN, J. HOPPER. JR.. J. L. HODGES. JR.. O. E.GUTTENTAG, K. G. SCOTT, I. XN. TUCKER axo B. BRADLEY (From the Department of Medicine and the Radioactivity Research Center, Univernty of California School of Medicine, San Francisco, Cal., and the Department of Stanstics; Oniversity of California, Berkeicy, Cal.) (Submitted for publication July 15, 1958: accepted March 5, 1959) (16, 17). However, it has not been shown that predictions based on total body mass are less ac- curate than those based on lean body mass, which requires a separate measurement (15, 16). Consideration of fat thickness and girth measurement, in addition to height and weight, was found by * This investigation was supported by research grants CH-1271 and G-3666) from the Insututes of Health, United States Public Health Service. from the James W. Edwards Memorial Fund (allocated by the Committee on Research of the University of California School of Medicine}, and from the American Heart Association and the San Francisco and Monterey County Heart Associations. t Research Fellow of the American Heart Association. relate the volumes to height and weight and a graphic system for their rapid application to prac- tical problems has been developed. Byanaivsis of individual differences, ‘residuals,’ from the mean regression platress—~we have studied the effects of the following factors on the variability of the data: age, bodybuild, habits of physical activity and, to a limited extent, seasonal changes. omen wc. The-subjects were stlected by careful screening from normaly active volumecrs. Most:of them were white (see Table I for racial and nationat-origins). Informa- tion from the prison heaith recordswas supplemented by interviews conducted by # physician from our group, by miniitm X-rays of the Bhest and by laboratory tests which included complete flood count, sedimentation rate, urmalysis, electrocardiogcam and a serologic test for syphilis. Volunteers were rejected if the results of these tests were abnormat, if their blood pressures were abose 140mm. Hg systolic or 90 diastolic, or if they had recognizable disease or pgst history of illness, such as rhewmatic ‘fever or tuberculosis, which might be present Rad el Ltd 1065 C4 |e 2SUByEcTS - Tote ratty of Caiforn presumably results from differences in body composition, since blood volume correlates both with body density (5, 15) and with lean body mass have Been derived for the plane surfaces which siting from ihe regardless of methods employed, when values for whole blood volume (Vwb), red cell volume (Vrbe} and plasma volume (Vpl) are related to body weight, height, or combinations of these measures (1-5, 13, 14}. Some of this variation IY GPOn CON. A rather large scatter of data around mean prediction values has been found byall workers, eOroduce MT is tively few or not entirely healthy subjects (7-13). Divergent results have been reported concerning the influence of age and ot physical training on blood volume (2, 18-23). Wetravevused the Cr* method of Sterling and Gray (24,23) to measure Vrbe and, indirectly from the-hematocrit, Vwb and Vp! in 201 healthy prison inmates. Trivariate regression equations FOPeENCE Uo One own control data, based often on study of rela- not in the middie ranges. ther ise. ags1on Fleets fa variance encountered among healthy subjects. Most clinical investigators have collected their Hicks. Hope. Turnbull and Verel to. improve prediction (14). Gregersen and Nickerson (3) classified subiects according to somatotvpe and found that variance of blood volume per unit ct body surface area was reduced to an important degree when dealing with extreme body types but Oped HOM Obes Methods employing radioactively tagged.red cells have been widely adopted for measurement of blood volume. However, in comparison with the work which has been done with the dve (1-5) and carbon monoxide (6) methods, little artention has been given to the establishment of mean values for men and women or to study of the