METHODS

ALl samples were collected in 12 ml vacutainers (Becton-Dickinsan)
with an ACD anticoagulant. - The samples were shipped by air, on ice, from
“| Kyvajalein Atoll, Marshall Islands to Honolulu, Hawali for transshipment to Ana Arbor.

Washed xed bivod cells and plasma were stored

at -70° ¢ prior to typing.
The conditions for electrophoresis and typing.of systems 1, 2, 8, 9,
10, 12, 13, 15, 16, 18 and 20~24 in Table 1 were carried out as described
previously (2).

Electrophoresis of systems 14 and 19 and staining of

14 omployed the method of Spencer, Hopkinson and Harris (3), and staining
' far systen 19 employed the positive staining method as reported by Peters,
Hopkinson-and Harris (4).
Charleswocth

System 3 was determined by the metnod of

(5), system 4 by the method of Tashtan (6), system 5 by

the methed of Chen, Anderson and Giblett (7), system 6 by the method of
Weltkamp (8), system 11 by the method of Edwards, Hopkinson and Harris
(9), and system 20 also by the methods of Wei tkamp et al (10) and Yanis
et al (1).

FINDINGS

le The polymorphisms.--Genetic polymorphisms were observed in six
of the systems:

haptoglobin, phosphoglucomutase-1, adenosine deaminase,

acid phosphatase, 6-phosphoglucose dehydrogenase, and group specific

PB

aa.on)

50

=

Ree|

Phenotype and allele frequencies are given in Table 2.

2
o-

component.

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